How embarrassing... This afternoon I created a 2L yeast starter for a Sierra Nevada clone. I took my White Labs California Ale WLP001 vial out of the fridge a couple hours prior to pitching it. Vigorously shaking the yeast to mix up the liquid with the cake, I proceeded to unscrew the cap, resulting in a volatile eruption of yeast spewing from the vial. Sad thing is I knew better to wait for it to settle antecedent to opening. Although only half the vial make it to flask, I'm certain with enough TLC, I'll rebuild the colony by Sunday (brew day). Has anyone else suffered from the same fate?