Aeration/oxygenation after fermentation starts

Discussion in 'Homebrewing' started by mattbk, Mar 27, 2014.

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  1. mattbk

    mattbk Savant (1,111) Dec 12, 2011 New York

    Brewed a 1.086 OG English Strong Ale 12 days ago. Added about 400 billion yeast cells for a 5 gallon batch, which works out to about 1 mil cells / mL / deg P.

    I don't have any means of pumping in O2 into the wort. Usually, this isn't a problem, as I don't do many high gravity beers, and I pour beer through a strainer into my fermenter, which I believe provides a good amount of oxygen. But, while I did realize I'd probably need to pump some in for this high gravity beer, I did not have time to purchase any such assembly and hoped for the best. Pitched at about 60 deg F, allowed to warm to 62 to 64 deg F.

    Checked after 10 days and gravity at 1.040, target is ~1.020. I'm warming it up to 68 deg F and I expect after another week it will be close to finished with primary. However - I contemplated adding in O2 at this stage. I won't - but I did contemplate it.

    I am figuring if there is still maltose left in solution, a boost of O2 would probably start the yeast multiplying again. They would grow, then quickly consume all the O2, and this might finish the job faster. I can't think of anything wrong with this, except the fact that it defies conventional wisdom about adding in oxygen once fermentation begins. Again - I'm not going to do this - but can anyone think of a (scientific) reason why adding some oxygen mid-fermentation would be a bad thing? I'd appreciate your thoughts.
     
  2. DrewF

    DrewF Initiate (0) Jan 3, 2010 Pennsylvania

    I think the issue is that oxygen uptake occurs during the exponential growth phase and in this example the yeast should be close to maximum population density already.
     
  3. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    Matt, I personally would have some concerns about adding oxygen at this point:
    • If the introduction of oxygen does not ‘stimulate’ the yeast to continue fermentation you know have greatly oxidized beer.
    • If the yeast does get ‘stimulated’ but the amount of oxygen is greater than what the yeast can take up, you have oxidized beer
    I think your instincts of: “I'm not going to do this” is a good call.

    Cheers!

    Jack

    P.S. I do not think that having an English Strong Ale ending up at 1.040 is too bad of a thing. I am contemplating brewing a beer like Hill Farmstead Everett (which is an OUTSTANDING Porter); Everett has an OG = 1.088 and FG = 1.030. Having some body in your beer can be a good thing!
     
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  4. ericj551

    ericj551 Pooh-Bah (1,638) Apr 29, 2004 Canada (AB)
    Pooh-Bah

    I think 1.040 is pretty high FG for an English Strong ale. It's barely over 50% apparent attenuation, where as your example is 65%. If the beer tastes good, I would go with it, but I suspect the OP's beer is pretty sweet at this point.

    I agree with your advice not to add O2, personally I would try gently rousing and give it another week. If that doesn't work I would build up a decent starter and pitch while it was active. I did this on a BSDA that stalled around 1.030 and I just bottled it at 1.010.
     
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  5. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    What DrewF said. BTW, oxygen doesn't stimulate yeast to do attenuation. It's a component for building sterols so that the cell walls can remain strong/flexible after dividing.
     
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  6. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    Matt, @ericj551 has some good suggestions. If you think your beer is too sweet for your liking, then try the rousing/pitch growing yeast approach.

    Cheers!
     
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  7. Applecrew135

    Applecrew135 Crusader (431) Jul 18, 2012 Pennsylvania

    I can't remember where I read this, but IIRC, if you are going to add additional O2 for a high gravity brew, it should be done within 24 hours of pitching. Later than that you run the risk of oxidizing your beer. I think this is also embodied within the "Texas Two-step" fermentation, where the first step creates a large starter for an equal addition of wort the next day.
     
  8. mattbk

    mattbk Savant (1,111) Dec 12, 2011 New York

    Hmm. This is where I disagree. My guess is that growth is limited by oxygen when making beer. More oxygen would have yielded higher growth, and thus more yeast cells to complete the fermentation once the oxygen ran out. Compare beer fermentation to a stir plate starter, where oxygen is constantly being supplied. Here growth is (presumably) limited by available sugar ie starter size.

    I'm not saying the oxygen will lead to attenuation. I'm saying the oxygen will lead to growth, and the increased cell count will lead to attenuation. Although admittedly, high gravity beers need stronger cells to overcome the high osmotic pressure and high alcohol generated, so oxygen is helpful here too.

    Again, I am thinking of fermentation as an oxygen limited process - which is true, as it is anaerobic. Another way to think about it - if I figured out the amount of O2 to feed a 1.040 beer, and fed this to my current fermenting beer, is there really a risk of the yeast not taking it up?

    The beer is going down the right path, and does taste good, but would certainly benefit from further attenuation. I know in this case that this high FG isn't a function of recipe or mash conditions. That being said - I am the last person to say that a beer with a high FG is necessarily "overly sweet". (Good example - Everett; Better example - Lagunitas Sucks)
     
  9. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    Matt asks: “Another way to think about it - if I figured out the amount of O2 to feed a 1.040 beer, and fed this to my current fermenting beer, is there really a risk of the yeast not taking it up?”

    I will be perfectly honest that I have no idea here. I personally do not have the skills to figure out the amount of oxygen to feed 1.040 beer. I also do not understand what exactly makes yeast cells stall the fermentation process. Do you think that all of the remaining yeast cells in your 1.040 beer are healthy/viable and that the only thing that is stopping them from finishing the job is lack of oxygen?

    Cheers!
     
  10. billandsuz

    billandsuz Pooh-Bah (2,097) Sep 1, 2004 New York
    Pooh-Bah

    ten days is probably not so long to worry. with an OG of 1086 I would expect a long time to FG. so far, it is just as likely that the yeast are slow but not stalled.

    if it were me, I would not oxygenate. let the bugs do their work. maybe rouse them a bit, a slight bump in temp probably wouldn't hurt either this far out either. but too soon to think about doing something to address an issue that might not even exist. my advice - let it ride.
    Cheers.
     
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  11. mattbk

    mattbk Savant (1,111) Dec 12, 2011 New York

    Let me back up a bit. When we calculate pitch rate, understand that if I pitch 400 billion cells into wort, I get substantially more than that in the beer during fermentation. Just as in a starter, feeding the cells oxygen and sugar will make them multiply.

    In beer, I believe the lack of oxygen stalls the growth, and metabolically shifts the yeast to begin anaerobic (or no oxygen) fermentation.

    So - while yes, I do think that the yeast in my beer is probably fairly viable - if I had MORE viable yeast they would finish the job quicker. By feeding them oxygen I am theorizing I might be able to get them to multiply, and complete the job a bit faster - at a minimal risk to the beer, as the exposure of the beer to oxygen will be fairly short (during the growth phase only).

    EDIT: @VikeMan's Brewcipher will estimate amount of O2 to feed a beer based on batch size, type, and gravity. Perhaps he can share where his model was derived from.

    This is a discussion in theory only. I am not going to be doing anything I am postulating. But, I do think the method I'm suggesting may have some merit.
     
  12. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    @mattbk, a point of clarification: are you of the opinion that your beer is just ‘slowed’ at this point in time (vs. stalled)?

    Cheers!

    Jack
     
  13. mattbk

    mattbk Savant (1,111) Dec 12, 2011 New York

    yes, slowed only.
     
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  14. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    @billandsuz wins the Gold Medal: “my advice - let it ride.”

    Cheers!
     
  15. FarmerTed

    FarmerTed Pundit (928) May 31, 2011 Colorado

    If all else fails, add a vial of Brett C and re-name it an old stock ale.
     
  16. DrewF

    DrewF Initiate (0) Jan 3, 2010 Pennsylvania

    Just pulled out my Yeast book and oxygen uptake actually occurs during the lag phase. Otherwise the book specifically mentions that a second dose of O2 for big beers can be made 12-18 hours after pitching, to allow the yeast to divide at least once. If you have it there's good reading starting at page 75, but I found no specific mention of the effect of adding O2 during later lifecycle stages.
     
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  17. TimoP

    TimoP Initiate (0) Oct 19, 2011 Pennsylvania

    So, you're contemplating adding O2, which you think would stimulate multiplication of the yeast, in order to reach your FG faster. But if that's the goal, why not just add more yeast? And not risk potential oxidation. This of course assumes that having more yeast is the answer to your problem.

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC152411/
    This study found that adding O2 during the stationary phase of fermentation resulted in an increase in fermentation, but cell population was not effected. However, thoughts on potential oxygenation weren't covered in the study. But it seems to me, that adding a small dose of O2 would not negatively effect fermentation and it would be consumed by the yeast.
     
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  18. mattbk

    mattbk Savant (1,111) Dec 12, 2011 New York

    Yes, you are correct. Adding more yeast absolutely makes more sense than feeding the cells oxygen. This was a theoretical question that I think was answered by this passage:

    "We demonstrated that a pulse of dissolved oxygen (1 to 10 mg liter−1) can stimulate alcoholic fermentation.... However, such an oxygen pulse stimulates fermentation only moderately but finally has an influence on long-term fermentative capacities."

    Thanks.
     
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  19. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Trial and error mostly. Discussion here. Also, the formula in that thread is for my usual batch size (5.1 gallons), but in BrewCipher, it is of course adjusted proportionally up/down for batch size.
     
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  20. honkey

    honkey Maven (1,350) Aug 28, 2010 Arizona
    Trader

    Adding oxygen this late in the game will encourage fusel alcohol production and ester production. Since the yeast has been stressed, you already will have increased production of esters and fusels. Next time you get into a situation like this, I would recommend trying the olive oil or safflower oil method to give the yeast the unsaturated fatty acids it needs.
     
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