Coagulation of protein and albumin -- how to ensure clean hot break

Discussion in 'Homebrewing' started by herrburgess, Apr 25, 2015.

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  1. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Do you guys utilize step/infusion mashing, or perhaps even decoctions (if not in a professional/commercial setting, then maybe in a home setting)? If so, have you noticed any differences like the one I mentioned above?
     
  2. bergbrew

    bergbrew Initiate (0) Jan 12, 2004 Minnesota

    We use step, but I think I'm misunderstanding the question. Your concern should be with the pH of the wort, not the mash. IIRC, the isoelectric point for proteins is quite low (around 4.9 or so). Usually people try to get around 5.2 or so. Many German brewers will acidify the wort during the boil (like maybe 30 minutes from the end). You don't want to do it at the beginning, as hop utilization drops with a lower pH.
     
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  3. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    “I do many/all of the same things to obtain clear wort, but recently have had some batches where my existing processes didn't seem to result in as much coagulation of proteins during the early stages of the boil. What do you do in such cases (assuming you have encountered them)?” In all of the 350+ batches of beer that I have brewed I have never experienced a non-substantial hot break. In fact I have the ‘opposite’ problem where I have to watch the kettle like a hawk to make sure I don’t experience a boil over.

    “There are also the factors of pH that may be playing a role here, as discussed. Just thought I'd ask about the protein rest since, especially when I have utilized one as part of a decoction regiment, I have noticed a distinct layer of coagulated protein atop the grain bed before (and during) sparge.” I have no way of knowing for a fact that your issue is due to not having a proper boiling wort pH but I am pretty confident that the ‘solution’ is not the need for a protein rest. I generally do not conduct protein rests in my hombrewing practice since I do not have a need for that aspect. For the sake of completeness I will state that I conducted a step mash which included an acid rest, protein rest and a starch conversion when I brewed my Grodziskie but I only did that since that batch was 100% Wheat Malt.

    Cheers!
     
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  4. jamescain

    jamescain Initiate (0) Jul 14, 2009 Texas

    I've noticed greater clarity in by wort by upping my Ca level to around 100 ppm and making sure my boil pH is around 5.3-5.5 at the start of the boil. A good strong boil seems to help as well. I usually target anywhere from 5.2 to 5.5 for a mash pH depending on the beer and I treat my sparge water with acid so that it doesn't increase the pH too much.

    I skim only because it gives me something to do and it helps prevent boil over, which is especially helpful when trying to boil 14 gallons in a 15 gallon kettle.
     
  5. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    I think I found my issue. My water calculator was giving me a 'final mash pH' of between 5.4 and 5.5. I have been taking the pH readings at mash out, and assuming the 5.5 I was getting was going to drop to 5.2 or so at room temp. Without a pH meter (just using Precision Labs pH strips), I was unable to get a good reading at room temp. Now, a closer look at my water calculator reveals (in fine print) that the 5.5 I was getting *is* at room temp. Ergo, my pH has been slightly too high at the start of the boil. That said, the advice to add a couple teaspoons of Calcium Chloride during the early stages of the boil didn't help. I'm now going to add a bit more acidulated malt to get to the 5.2 range and see if that does the trick. Thinking back, I did add more acidulated than I had calculated to the last batch where proteins coagulated better, just because the HBS sent us a bit more. We will see...and thanks for all the advice. If this sounds like I'm on the right track, please let me know.
     
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  6. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    According to Kai Troester: “Germans love step mashing and the Hochkurz mash is the most popular mashing schedule today.”

    The Hochkurz mash has two steps:

    · 63 °C (145 °F)

    · 72 °C (162 °F)

    You can clearly see that a protein mash is not a part of the Hochkurz mash.

    Cheers!
     
  7. telejunkie

    telejunkie Savant (1,107) Sep 14, 2007 Vermont

    great discussion! sorry, just saw this thread. At this point seems like I have little to add to the discussion beyond what has already been stated. Herrburgess, the above post makes me think that mash pH will help the situation. I noticed a year or two ago that my hot break on lighter colored brews really started to jump out when I started adding phosphoric acid to my mash & sparge water which brought my mash pH & kettle pH to a much better spot since i have hard water. Seriously...i thought the strong hot break in my porter's & stouts had to do with something in the darker grains. Actually i have yet to skim...but may try this weekend since my plan is to brew a german pils....

    As for the German's, the brewers are pretty tight-lipped so I get all my info about them as basically here-say...but it was my understanding that the protein rest has been all but relinquished to annals of mashing history with a few exceptions..although still many perform a step mash. I could be wrong...especially with hefe production. I personally still perform a multi-step mash and try to decoct at least once when brewing my hefe. I don't know of any tests that have been shown performing a protein rest will result in a thinner, more watery beer...I do know that there are still homebrewers who perform a protein rest and are willing to shout from the top of tall buildings that it does not lead to a thinner beer. Also its effects on head retention...? What I probably should do is to pass this along to Ashton Lewis, as this is his department.
     
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  8. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    Dave,

    It would be interesting to see what Mr. Wizard has to say on this topic.

    Cheers!
     
  9. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    I got the info about protein rests from Steve Holle's presentation. Where did the info about the thin, watery beer come from?
     
  10. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    As I posted:

    "Below is what John Palmer states in his online book How to Brew:

    “Moderately-modified malts benefit from a protein rest to break down any remnant large proteins into smaller proteins and amino acids as well as to further release the starches from the endosperm. Fully-modified malts have already made use of these enzymes and do not benefit from more time spent in the protein rest regime. In fact, using a protein rest on fully modified malts tends to remove most of the body of a beer, leaving it thin and watery."

    Cheers!
     
  11. hopfenunmaltz

    hopfenunmaltz Pooh-Bah (2,647) Jun 8, 2005 Michigan
    Pooh-Bah

    My advice is to get a good pH meter. You are making 1 bbl batches, why screw around with the pH strips.
     
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  12. bergbrew

    bergbrew Initiate (0) Jan 12, 2004 Minnesota

    Narziss: "Qualitative and Quantitative aspects of Mashing ", Brauwelt, Vol. III, 1993
    Kunze: Brewing and Malting

    IIRC, Narziss called the character "hollow" or something like that.
     
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  13. ElmerTLee

    ElmerTLee Initiate (0) Apr 25, 2015 Connecticut

    Relax, have a homebrew...sorry I had to.

    I recently stopped the skimming process. I don't think big breweries do it, so why should I?
     
  14. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Thanks. Searched for the full text of that article and couldn't find it. Do you, or anyone else, have a copy. German would work, too.
     
  15. bergbrew

    bergbrew Initiate (0) Jan 12, 2004 Minnesota

    I used to have one somewhere in my office, but I moved a while ago. I'll see if I can locate it.

    Edit: It's also referenced in "An Analysis of Brewing Techniques," which is where I recall reading it first (although I probably saw it on HBD back in the day as well). I also, unfortunately, seem to have misplaced that book as well
     
    #55 bergbrew, Apr 30, 2015
    Last edited: Apr 30, 2015
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  16. koopa

    koopa Initiate (0) Apr 20, 2008 New Jersey

    What temperature are you sparging at by chance @herrburgess ? I ask because using sparge water over 170F increases the risk of dissolving beta glucan into your wort. It is my understanding that a protein rest can indeed activate protease enzymes that aid in breaking down beta glucan. Protease enzymes will help minimize the solubility of polypeptides responsible for beer haze.

    NOTE: This reply is merely with regards to beer clarity in general and your questions about whether a protein rest can encourage better clarity. It has nothing to do with your lack of hot break!
     
  17. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    You mean "sparge water"? Suspect that was a typo, but just checking.
     
  18. koopa

    koopa Initiate (0) Apr 20, 2008 New Jersey

    yup.... corrected!
     
  19. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Generally it is slightly above 170. I will try getting it closer to 170 next time to see if that helps. Thanks for the tip.
     
  20. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Cool. Don't worry too much if you can't find it. I trust what you are saying, but I keep hearing that article referenced, and so I'd like to give it a read. Still have lots to learn....
     
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