A Dry Yeast Rehydration Experiment

Discussion in 'Homebrewing' started by pweis909, Jan 5, 2019.

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  1. pweis909

    pweis909 Grand Pooh-Bah (3,250) Aug 13, 2005 Wisconsin
    Pooh-Bah

    Some new information from Fermentis on pitching dry yeast prompted me to experiment.
    You can find discussion of the new Fermentis pitching recommendations here:
    https://www.beeradvocate.com/community/threads/pitching-yeast-in-low-ph.599478/#post-6311011

    Method
    I did a split batch to compare the Fermentis dry yeast sprinkling approach to the approach I take when I rehydrate dry yeast. The base beer is a low gravity IPA. I used an 11.5 g pack of US-05. The recipe is at the end of this post.

    I evenly split 4.5 gallons of 1.056 OG wort that was mashed at 148 degrees into two stainless steel fermenters. When the fermenters had received 1.5 gallons, I stopped filling to weigh out and sprinkle half the pack of dry yeast into one of the fermenters, then resumed filling. When done filling, each vessel had 2.25 gallons of wort. At that point, I rehydrated and subsequently pitched the remainder of the yeast packet.

    My rehydration approach used boiled water, cooled to 95 F. Half a pack of yeast (pre-weighed) was added to a quarter cup of this water (about 10 mL per gram of yeast). After 15 min, the yeast was gently stirred to make a more uniform slurry and allowed to rest another 15 minutes before pitching. The yeast temp was 70 F at the time of pitching.

    I fermented without active temperature control. The adhesive thermometers on the fermenters showed 64 F at the time of pitching and rose to 66 F during fermentation. On the fifth day after pitching, I sampled each wort and measured gravity with a hydrometer.

    Results
    Before one full day elapsed, the fermenter with the sprinkled yeast appeared slightly more active in terms of krausen and airlock activity, but by 36 hours, no discernable difference was observed. On the fifth day after pitching, I measured no airlock activity or krausen was observed. The gravity of the beer produced by the sprinkled yeast was 1.005 and the gravity of the beer produced by the rehydrated yeast was 1.007.

    Discussion
    2 gravity points is nothing to get hung up about. I deem these to be close enough as to not really be distinguishable from a sensory perspective. I tasted each an couldn't distinguish anything different. I was surprised, given the high attenuation, that both beers still had some perceptible sweetness, but it did not seem to interfere with my enjoyment of the hoppy goodness.

    This experiment supports the idea that sprinkling dry yeast is a viable option when using a Fermentis packet in a moderate gravity wort. It didn't seem to have any impact on my beer. Perhaps the pitch rate is high enough that rehydration doesn't matter. Maybe the impact of rehydration would be observed if the pitch rate were lower or the gravity of the wort higher?

    In the past, I have both sprinkled and rehydrated, but not in side by side experiments. I anecdotally observed that sprinkling yeast appeared to cause fermentations to take longer to start. I always had sprinkled on top of the wort, and in this experiment, I followed my understanding of the Fermentis approach of sprinkling before the fermenter received all of the wort, adding yeast after 2/3 of the wort volume was reached and then cover the yeast with the remaining wort. Perhaps this is an important difference? It would mix the yeast into the wort a little more than sprinkling on top. In any event, either approach to sprinkling yeast is easier than rehydration.

    My plans for this batch is re-combine into one keg after another week. No point in trying to maintain these as separate beers, to my way of thinking.


    Recipe
    Sightless Sow (an APA-AIPA tweener, harkening back to the good old days of IPA)
    4.5 gallons, BIAB approach
    Pale bitter water profile from BruNWater

    Ingredients
    6 lbs 4 oz US 2-row pale malt (Great Western Premium)
    5 lbs 3 oz Maris Otter (Muntons, I think)
    4 oz Caramalt (Thomas Fawcett)
    0.5 oz CTZ (13.1% AA) at 75 min
    1 oz Cascade (6.3% AA) at 15 min
    1 oz Centennial (9.1% AA) at 15 min
    1 oz Cascade (6.3% AA) steeped at 170 F for 20 min
    1 oz Centennial (9.1% AA) steeped at 170 F for 20 min
    1 pkg Safale US-05
    1 oz Amarillo (9.2% AA) dry hop in primary for 5 days contact time
    1 oz Simcoe (13% AA) dry hop in primary for 5 days contact time
    1 oz Centennial (9.1% AA) dry hop in primary for 5 days contact time
    1 oz Cryo-Cascade (12% AA) dry hop in primary for 5 days contact time

    Mash for 90 minutes at 148
    Fermentation temp started at 64 F, rose freely to 66 F by day 2, as per stick on thermometer. No active temperature control was used.
     
    #1 pweis909, Jan 5, 2019
    Last edited by a moderator: Jan 7, 2019
  2. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Sounds like another potential experiment, to compare the two "sprinkling" methods. And here's a third: A pro brewer I know puts dry yeast in the bottom of the fermenter, then racks the wort onto it, with all the agitation that naturally comes along with that.
     
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  3. Maestro0708

    Maestro0708 Initiate (0) Feb 27, 2015 Kentucky

    Thanks for sharing. I will give this alternate sprinkling method a whirl tomorrow when I pitch US-05 into my Oatmeal Stout. FWIW, i havent been rehydrating as of late (since I noticed the yeast manufacturer's pitching instructions on the packaging simply recommends "sprinkling onto wort") and personally havent noticed increased lag time between pitching and signs of fermentation.
     
  4. skivtjerry

    skivtjerry Pooh-Bah (1,865) Mar 10, 2006 Vermont
    Pooh-Bah

    That's how I do my beers with dry or liquid yeast - rack the wort onto the yeast in the carboy.
     
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  5. HerbMeowing

    HerbMeowing Maven (1,295) Nov 10, 2010 Virginia
    Trader

    Two observations:
    1) The known unknown: "Maybe the impact of rehydration would be observed if the pitch rate were lower or the gravity of the wort higher."
    2) The unknown unknown
    3) n = 1

    Bottom line: more yeast ... more better
     
  6. pweis909

    pweis909 Grand Pooh-Bah (3,250) Aug 13, 2005 Wisconsin
    Pooh-Bah

    I think more healthy yeast is a good hypothesis for why sprinkling works, given that rehydration in wort is said to create osmotic stress. Dry yeast packets contain lots of cells, so you can get away with sprinkling. Of course n=1, so maybe that is a false assumption, but I think enough folks report sprinkling and "getting away with it" that we can be comfortable with the idea. In any event, I'll probably stop harassing people to rehydrate, as I have done in the past.

    Since I use dry yeast quite a lot and can fairly easily split fermentation into two small batches, I'll probably continue to see if I can uncover some of the limitations vis a vis pitch rate and gravity and possible variations in behavior among different strains. Based on the info from Dr. Cone that @JackHorzempa posted in the linked thread in the first post, I think it is fair to say that the yeast labs would have already explored all of the ideas I have in my mind, but they don't always publicize stuff, and, well, I sort of like puttering around with my 5 gallon brewhouse.
     
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  7. HerbMeowing

    HerbMeowing Maven (1,295) Nov 10, 2010 Virginia
    Trader

    What I was thinking .. as well.
     
  8. minderbender

    minderbender Initiate (0) Jan 18, 2009 New York

    I'm a fan of this kind of experimentation and I hope you continue to post your results. I'm not in a position to experiment much along these lines, but if I find myself brewing a suitable batch I'll try to do something similar. Also, to be clear, I don't have a dog in this fight. I would simply like to know what the effects of rehydration are so that I can make an educated choice on brew day.

    That being said, two points made in the passage I've quoted above seem important to me. First, as you noted, the pro-rehydration argument is that wort puts osmotic stress on the yeast. (In brief, the idea is that dry yeast need to draw water into their cells from the environment, and a higher gravity basically requires the yeast to pump water "uphill"—that is, to pull water across a membrane despite the fact that water might naturally "flow" in the other direction.) That's why I was puzzled that you rehydrated in wort (if I'm reading your original post right)—is that really "rehydration" in the sense that its advocates use the term? I would also observe that osmotic stress is presumably a function of the gravity of the wort. 1.056 is pretty middle-of-the-road. If you wanted to push the boundaries, you might try a considerably higher gravity.

    Which brings me to my other observation. The claim that rehydration proponents make is that rehydration kills fewer cells than sprinkling directly onto/into wort. It would generally be acknowledged that this can be counteracted by adding a surplus of cells, which I think is what rehydration proponents would argue you have done here. Another way of putting it is that good homebrewing practices call for pitching a comfortable cushion of "extra" yeast (that is, a cell count well beyond what is strictly necessary to ferment the wort), and you may not observe much benefit from rehydrating until you have depleted this cushion. This makes it inherently difficult to run experiments, because you have to push the envelope to get into situations where rehydration would really shine.

    I think the best version of the anti-rehydration argument basically says: That's exactly right, you should never be pushing the envelope in terms of cell count and so you should never really need rehydration. If you are pitching an appropriate number of cells into your wort, then there are enough cells that losing x% to osmotic shock will not impede a healthy fermentation. Rehydration saves x% of cells at the expense of a lot of extra effort and/or increased risk of infection (that is, either you are going to considerable effort to create a pint or two of sterile water, or you are risking the introduction of other microbes), and this is a bad tradeoff in most realistic scenarios homebrewers will face. You should always just pay an extra $y and pitch a little more yeast in any realistic scenario where cell count is in question.

    Of course it depends on what x is, and it depends on how difficult you find it to sterilize a pint or two of water and warm it to the correct temperature. But the idea that rehydration is always called for is probably wrong.
     
  9. pweis909

    pweis909 Grand Pooh-Bah (3,250) Aug 13, 2005 Wisconsin
    Pooh-Bah

    Wow, surprised no one else caught that. My mistake in the original post.
    First sentence of paragraph 3, boiled water, not boiled wort, cooled to 95F, was used to rehydrate.

    I wish I were a better proofreader, or better yet, I wish BA would let you edit posts beyond 15 minutes.
     
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  10. pweis909

    pweis909 Grand Pooh-Bah (3,250) Aug 13, 2005 Wisconsin
    Pooh-Bah

    Thanks to our moderator for fixing the typo for me.
     
  11. Brewday

    Brewday Zealot (721) Dec 25, 2015 New York

    I also do this and sometimes i will add a small amount of yeast in the kettle when it's cooled before it goes in the fermenter.
     
  12. jeebeel

    jeebeel Zealot (667) Jun 17, 2003 Texas

    @pweis909, this is very interesting. Thanks for posting it.
     
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  13. pweis909

    pweis909 Grand Pooh-Bah (3,250) Aug 13, 2005 Wisconsin
    Pooh-Bah

    Probably will add to it soonish
     
  14. mikehartigan

    mikehartigan Maven (1,421) Apr 9, 2007 Illinois

    I've been waiting 10 years to say I told you so! :stuck_out_tongue_winking_eye:
     
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  15. pweis909

    pweis909 Grand Pooh-Bah (3,250) Aug 13, 2005 Wisconsin
    Pooh-Bah

    Your patience has been commendable!
     
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  16. pweis909

    pweis909 Grand Pooh-Bah (3,250) Aug 13, 2005 Wisconsin
    Pooh-Bah

    Experiment #2. Almost the same deal, using Fermentis BE-256 in a Belgian pale ale. Again, a single wort was split into 2.5 gallons, with half a pack of properly rehydrated yeast pitched in one, and sprinkling non-rehydrated into the other. I unintentionally deviated from the Fermentis instructions of sprinkling dry yeast into half the wort and then topping it with the remaining wort. I forgot about that aspect of the first experiment until I had the wort evenly split into fermenters, so I just sprinkled the old fashioned way, directly on top of the wort. I did this during the 30 minutes that the remaining yeast was rehydrating. I'm expecting the same result as before: both ferment to comparable FG and no perceptible difference will be detected.

    Norval, an Orval-inspired beer brewed in the Northwoods. Don't get too wrapped up comparing to some pre-existing clone recipes; I didn't go out of my way, and neither should you.

    OG: 1.056 Calculated
    IBUs: 45 (calculated)
    SRM: 5
    5.5 gallon batch (2.5 gallons per fermenter; avoided the trub-heavy remaining half gallon)

    Grains and Fermentables
    8 lbs Best Heidelberg Pilsner Malt
    2.5 lbs Avanguard Vienna Malt
    0.25 lbs Muntons Caramalt (10-14L)
    1 lbs Golden Candi Syrup (5L) (added post boil)

    Hops
    0.75 oz Pekko, 15% AA, 60 min (34.8 IBU)
    0.5 oz Crystal, 3.8% AA, 15 min (4 IBU)
    0.5 oz Styrian Celia, 4.5% AA, 15 min (4.8 IBU)
    1 oz Styrian Celia, 4.5% AA, Whirlpool 20 min @170F (1.6 IBU)

    Dry hops
    (added second day of primary fermentation)
    1.5 oz Crystal
    0.5 oz Styrian

    1 Package Safebrew Abbey Ale BE-256

    Plan to package half in bottles with Brett clausenii and keg the other half without Brett, and taste side by sides periodically to observe the Brett impact. Yes, I realize this is not the strain that people typically use for Orval clones. Just what happened to be available when I was shopping.

    I'll report final gravity in a week or so.
     
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  17. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    "OG: 1.056 Calculated"

    For this moderate gravity ale my prediction is that both batches will ferment comparably (i.e., start and finish in comparable timeframes) and both will reach comparable final gravity values.

    I look forward to reading the results.

    Cheers!
     
  18. pweis909

    pweis909 Grand Pooh-Bah (3,250) Aug 13, 2005 Wisconsin
    Pooh-Bah

    My prediction as well. Numbers game argument. Most of my brews are below 1.060 OG, so direct pitch of dry yeast might never be a problem, assuming 10 to 11.5 g packets and 5 gallons of wort.

    BTW, that OG was measured with a hydrometer, not calculated. I measured it two ways, with a refractometer, calculating the SG, and also with a hydrometer. Not sure why I bothered to type calculated.
     
  19. dmtaylor

    dmtaylor Savant (1,149) Dec 30, 2003 Wisconsin

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  20. pweis909

    pweis909 Grand Pooh-Bah (3,250) Aug 13, 2005 Wisconsin
    Pooh-Bah

    I haven’t read the entire thread yet, but, yes, I have wondered about over pitching, specifically as relates to the Belgian styles I have made with dry yeast. One thought about this experiment was maybe the directly pitched yeast will develop more Belgian character because the pitch had fewer viable cells. I just posted in a different thread about some dry kveik yeast I am buying for which the supplier recommends a 1-g pitch to a 5 gallon batch to maximize the flavors everyone wants to generate with these yeasts.
     
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