Different math on yeast calculator

Discussion in 'Homebrewing' started by Scope4Beer, Mar 20, 2017.

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  1. Scope4Beer

    Scope4Beer Zealot (677) Sep 28, 2009 Pennsylvania
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    So I've noticed this discrepancy before but never thought too much of it, as it never seemed like a big difference. I use yeastcalculator.com to determine the size of my starters. It gives you the option when using a stir plate of either Zainesheff's or Troester's math. Z's equation always results in less yeast at finish compared to Troester's. Which one do you rely on and why?

    My current planned brew next weekend will be a 5 gal batch of a tripel with an OG of 1.074, which requires 255 billion cells. My WLP530 yeast was created 12/22/16, so currently has 47% viability per the website. Using Z's equation I would need a 3.2 L starter, but using Troester's it's only a 1.5 L starter (which would yield 257 billion cells). To put it another way, a 1.5 L starter with Z's equation would only yield 182 billion cells. This just seems significantly different.
     
  2. BeerMaverick

    BeerMaverick Zealot (718) Dec 14, 2010 Connecticut

    Yes, I've noticed this big difference as well - I really dug into the equations since I have my own brewing spreadsheet and wanted to know how to incorporate a starter calculator into it. Mr. Malty (Jamil's calculator) seems to be the most popular, but you're right that the starters are enormous (with my 2L flasks it always required either multiple flasks or stepped starters)... I went with Kai's stir plate equation in writing my spreadsheet because it seemed more reasonable in terms of starter sizes - it also matches BeerSmith's yeast starter calculator very closely. Despite Kai's and BeerSmith's smaller starter requirements I've gotten full attenuation every time, so I believe you're ok using these.
     
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  3. 1beerbaron

    1beerbaron Initiate (0) Mar 24, 2009 Ohio

    Are you adjusting the Mr. Malty calculator for the correct settings? Mr. Malty's seems to error on the side of slightly more yeast. It also is much more adjustable. This means that, if not adjusted correctly, it may give you wildly different numbers. I've found it to be only slightly larger than BeerSmith most of the time.
     
  4. Scope4Beer

    Scope4Beer Zealot (677) Sep 28, 2009 Pennsylvania
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    I don't use Mr. Malty, but rather yeastcalculator.com, which does incorporate Z's equation.
     
  5. honkey

    honkey Maven (1,350) Aug 28, 2010 Arizona
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    In all honesty, neither of them are going to be accurate, but they can sort of give you a ballpark idea.
     
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  6. PortLargo

    PortLargo Pooh-Bah (1,831) Oct 19, 2012 Florida
    Pooh-Bah

    When I had a homemade stir plate I found Zainesheff's figures (typically lower) most closely approximated my results. When I bought a commercial stirstarter it tilted more toward Troester's results (higher). The commercial stir plate had a little more "ommph" going on . . . I concluded the extra action did improve results. This is based on my personal experience doing multi starters over a period of years and measuring the actual quantity of yeast produced.

    The yeastcalculator site gives the option of using data based on two different researchers. Obviously the two models have different results which is probably to be expected in real world testing.

    More anomalies: If you use Brewer's Friends yeast calculator it also has two different stir plate growth options (White/Braukaiser) which are both different from yc's Zain/Tro . . . I draw the same conclusion that different models were tweaked/measured such that the results are different again. They even have a pretty good discussion on the models used. The errors in all of the above are magnified at lower innoculation rates (i.e. large starters). In a small starter (high innoc rate) the four models are pretty similar.

    Which one is correct? I believe @honkey hits the nail on the head . . . probably none are truly accurate. But after multi starters I've narrowed it all down to what produces the most accurate count for me, basically through a trail and error process. So let your entire fermentation process (including taste of beer) guide you to which sets of data points work best for your brew house.

    For @VikeMan , which model do you use in Brewchipher?
     
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  7. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Technically, neither. BrewCipher uses my own interpretation of the growth curves, which I suspect (but don't know) are the same growth curves Jamil used for his model. So BC's answers will generally be closer to Jamil's than Kai's. BC also uses some different assumptions about age related viability.
     
  8. Scope4Beer

    Scope4Beer Zealot (677) Sep 28, 2009 Pennsylvania
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    Thanks for the responses. I've always erred on the conservative side and gone with Jamil's calculations. Attenuation has always been as expected. In this case I may try Troester's, one to switch and up and see, plus this is a Belgian so I won't mind underpitching some if indeed that happens.
     
  9. honkey

    honkey Maven (1,350) Aug 28, 2010 Arizona
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    FWIW, I think that using a calculator at all is A LOT better than nothing. I feel that's necessary to say after having said the calculator won't be that accurate. What I would suggest if you want to get "proper" fermentation characteristics, is to start with the calculator and make adjustments as needed until your ales are hitting terminal gravity in 3-4 days or lagers in 8-12 days. The Mr Malty calculator doesn't take into account things like gravity of starter (I find 8 degrees plato to be the best gravity for starters at my brewery for the yeast strains I know well), temperature (I typically keep starters a bit warm... 24-26 degrees celsius), or individual yeast strain... some propagate faster than others. That said, I would start with the calculator results exactly as they say and fine tune from there to get the best results.
     
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  10. daem3384

    daem3384 Zealot (691) Nov 24, 2015 California

    I made an excel spreadsheet that uses the Monod equation with growth characteristics I've gathered from a few journal articles concerning sacch. cerv., and the numbers come out almost exactly the same as the Braukaiser numbers, so I think that is the model they use. Its a nice, simple microbial growth model, and it factors in the OG of the starter, but it doesn't integrate a lag phase or alcohol inhibition very well, so I always shoot for a 15% calculated over pitch.

    That being said, every yeast strain will have slightly different growth characteristics, so you'll never EXACTLY know how much yeast you're pitching without a hemocytometer (which would be a little pricey and unnecessary for the homebrew scale).
     
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  11. utahbeerdude

    utahbeerdude Maven (1,374) May 2, 2006 Utah

    Can you provide references for (or, better, links to) these journal articles? Cheers!
     
  12. daem3384

    daem3384 Zealot (691) Nov 24, 2015 California

    Fermentation Kinetics including product and substrate inhibitions plus biomass death: a mathematical analysis.
    Mathieu Bouville
    Institute of Materials Research and Engineering, Singapore (7 Feb 2008)

    Growth Kinetics of Suspended Microbial Cells: From Single-Substrate-Controlled Growth to Mixed Substrate Kinetics
    Karin Kovarova-Kovar and Thomas Egli
    Microbiology and Molecular Biology Reviews, Sept. 1998, pg 646- 666

    I also assumed perfectly spherical yeast cells with a diameter of 5 micrometers and a density of 1.1 g/cm^2.

    Lots of assumptions are made in these calculations, so a 15-20% error would be safe.

    I hope you like math!
     
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  13. utahbeerdude

    utahbeerdude Maven (1,374) May 2, 2006 Utah

    Thanks!
     
  14. honkey

    honkey Maven (1,350) Aug 28, 2010 Arizona
    Trader

    I'm working on setting up a small yeast propagation room in my house for Brett's and sour beers for work. I'll try to remember to do a few sacch starters with various yeast strains and share the results. I'll be using a hemocytometer for cell counts.
     
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