Froze yeast on accident

Discussion in 'Homebrewing' started by cawurm12, Jul 22, 2015.

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  1. cawurm12

    cawurm12 Initiate (0) Jul 22, 2015 Ohio

    Can I use frozen yeast (thawed) for my homebrew?

    So I froze my yeast by accident. I began brewing and found my liquid yeast frozen in my freezer. I removed from the freezer and thawed it as I was brewing. Once I put the beer in the primary fermenter, I decided to also add the yeast since it was back to liquid form. What should I do? Will the beer be OK or should I go get more yeast to add? Should I even add another packet of yeast or should I not open the fermenter? I have it in a clear carboy so I will be able to see if any progress is being made. So far, ~12 hours later, there are no signs of fermenting. Any suggestions or advice on what I should do?
     
  2. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    I always have a packet of yeast in my refrigerator in case of emergencies; a packet of US-05. Do you have something like this?

    I would suggest that you give your fermentation a bit more time (24-36 hours) to see if fermentation ‘kicks off’. If after that extended time you do not see signs of fermentation I would suggest adding some more yeast to the fermenter (which would be the packet of US-05 for me).

    Cheers!

    P.S. Dry yeast is perfect for emergency reasons since it last for years when stored in the refrigerator.
     
  3. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    At this point, since you already used it, I'd probably wait and see. Contrary to popular belief, yeast can survive freezing, at least some percentage of them.

    But...one concern could be underpitching. Was this a single vial/smackpack? Into what wort? If one pack would have been underpitching, you can bet that frozen/thawed yeast would be even more underpitched.
     
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  4. cawurm12

    cawurm12 Initiate (0) Jul 22, 2015 Ohio

    I'll give it a bit more time before I make any decisions. I used a smackpack of American Wheat 1010 for a 5 gallon batch. My buddy also spilled a very very small amount of the yeast while pouring (again, very small amount). Rookie mistakes, I know. Should I be concerned with underpitching? Would adding more yeast fix the potential underpitching issue? If so, how much should I add?

    After this fiasco, I do plan on stocking an emergency packet of yeast.
     
  5. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    5 gallons of what gravity?
     
  6. cawurm12

    cawurm12 Initiate (0) Jul 22, 2015 Ohio

    My gravity was in the 1.050-1.055 range.
     
  7. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    By most calculators, a single smack pack would be underpitching for your batch size and gravity. By the dumbed down instructions that appear on yeast packages/websites, it's plenty. That's before taking into account your spillage and freezing losses. Still, I would probably just ride this one out and see.
     
  8. cawurm12

    cawurm12 Initiate (0) Jul 22, 2015 Ohio

    Thanks for the advice. Hopefully it pulls through.
     
  9. premierpro

    premierpro Savant (1,060) Mar 21, 2009 Michigan

    Don't feel like your alone. I have also done this. I had to pitch more yeast. Good luck!
     
  10. DrMindbender

    DrMindbender Initiate (0) Jul 13, 2014 South Carolina

    Wild yeast survives freezing temps every year and still returns the following warm season. This selects for stronger individuals (at least freeze resistant) but will kill off a percentage of the yeast as well...natural selection at its finest. The real problem is that you don't know if the surviving yeast are more or less fit at reproducing and fermenting, which can't be assumed just because they survived through the temp extreme, and is the most important piece of info to determine if you have enough active yeast for adequate fermentation.

    So there will certainly be some viable yeast that survived the freeze, but the percentage of viable cells can't really be calculated, nor can the fecundity (reproductive success) of the yeast. That's why folks are telling you to let it ride out and see what happens first before pitching more yeast. You very well may have enough yeast to take care of this batch, and then again you may not...there really isn't a way to know for sure until you allow the yeast you pitched to get underway and do their thing.

    Did you use any yeast nutrient? This can help a little with underpitching or slow fermentation and can be added to the boil or in the fermenter for slow fermentation.
     
  11. cawurm12

    cawurm12 Initiate (0) Jul 22, 2015 Ohio


    I did not use any yeast nutrient. Just thawed the smackpack, popped it, and poured it in. How/when will I know if I need to pitch more yeast? Or if there is enough to get the job done? Or how much to add if I need to? Wait a few days to see if it is bubbling? If it is bubbling is it good to go or is there a duration/intensity of bubbling it needs to reach?

    Sorry for all the questions, and thanks everyone for the input!
     
  12. billandsuz

    billandsuz Pooh-Bah (2,097) Sep 1, 2004 New York
    Pooh-Bah

    If you are the kind that needs reassurance that your yeast are doing their thing you have a few options.

    Wait a few days, three max, and see if there is any activity. Bubbles in the airlock is not always the recommended indicator since gas can escape elsewhere while fermentation is occurring but for this purpose it is fine. Once you see bubbles you are making beer. The yeast is.

    Get another package of yeast, let it warm to the temp of the wort and pitch the yeast. You underpitched anyway so more yeast is recommended if you can swing it. And you'll feel better.

    Or pitch two packages of US-05. You do have US-05 right? The best, always works, classic, fool proof yeast. Get some.

    Cheers.
     
  13. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Bubbles (or lack of) are not a reliable indicator. Are you fermenting in a bucket? If so, open the lid, peek in, and see if a krausen has formed. I would wait maybe 48 hours from when you pitched to check.
     
  14. DrMindbender

    DrMindbender Initiate (0) Jul 13, 2014 South Carolina

    Are you fermenting in a bucket or in sobering you can see through? If it's see through, you can see the yeast actively fermenting and mixing the wort/swimming around while active fermentation is taking place. This is the indicator you want to see, along with airlock activity. Ultimately, you will need to take hydrometer readings to determine if you've reached final gravity...if after fermentation occurs and you haven't reached your final gravity, then I'd add more yeast.
     
  15. DrMindbender

    DrMindbender Initiate (0) Jul 13, 2014 South Carolina

    I don't see a need for 2 packs of 05...over the last 4-5 years of using 05 as my main yeast, I only use 1 pack for my beers that go up to around 1.060 OG and have never had any issues with underpitching. I think too many are a little obsessive about underpitching when it rarely occurs except in higher gravity beers. At least that's my observation after brewing for over 20 years.
     
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  16. cawurm12

    cawurm12 Initiate (0) Jul 22, 2015 Ohio

    My fermenter is clear so I'll be able to see what's going on inside. I'm fairly new to brewing, about 2 years now, on and off. I've always been under the impression you don't want to open up while fermenting.... Is this not true? I thought once you start the process and put the lid/airlock on you want to keep it sealed until it is complete. I've never opened up to check the gravity cause, as I just mentioned, I didn't think I was supposed to. If that's not a big deal, I'll be fine checking the gravity to see whether or not my yeast did its job and I'm where I need to be.
     
  17. DrMindbender

    DrMindbender Initiate (0) Jul 13, 2014 South Carolina

    If you see the described activity, let it ride...if you see nothing happening at all, then go ahead and add the 05. If no activity at all, then no need to check with hydrometer...if activity, wait until fermentation is complete as usual (krausen fall) and yeast settles in the bottom, then check gravity.
     
  18. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    One pack of US-05 (11.5 grams) would be about 230B cells, which if fresh is more than enough for 5 gallons @ 1.060, by just about anyone's reckoning.

    I think that would depend on what's defined as under pitching. In your example, and with the OPs batch, I don't know anyone who would say a pack of US-05 is under pitching.

    OP: An 11.5 gram pack of dry yeast contains more than double the cell count of a smack pack or vial.
     
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  19. cawurm12

    cawurm12 Initiate (0) Jul 22, 2015 Ohio

    Thanks again for the help. I'll let it ride and see if any action occurs.

    On another topic you guys are discussing....what are the pro/cons to dry yeast vs. the smack packs? I've used the dry yeast in the past and my local homebrew store recently convinced me to try the smack pack.

    Is there really much of a difference or is it just personal preference?
     
  20. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Somewhat personal preference. But also, liquid yeast is available in a staggering variety of strains. Dry yeast isn't.
     
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