German Lager Brewing Techniques -- Yeast Pitch Rate

Discussion in 'Homebrewing' started by herrburgess, Jul 4, 2015.

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  1. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    In a 2008 AHA presentation on German brewing techniques, the author says that German lager brewers typically pitch 15 to 30 million cells/ml. According to Mr. Malty, there are about 4.5 billion yeast cells in 1 milliliter of yeast solids, and that in a slurry only about 25% of the mass is yeast solids. Still this gives 1 billion cells/ml in a slurry. Assuming a viability even in the 50% range, you're still at 50 million cells/ml.

    What am I missing here? Is this a real disconnect, or are my calculations -- or is my thinking -- off somehow?
     
  2. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    Pitch rates generally are 'referenced' to the size of the batch and often times reference the gravity of the wort.

    The pitch rate often quoted by folks is the George Fix pitch rate for the case of repitching previously used yeast which is:

    · 0.75 million cells/ml/°P for ales

    · 1.5 million cells/ml/°P for lagers

    For the case of brewing a 12 °P lager this would yield 18 million cells/ml and once again this would be for the case of repitching yeast that was previously used to brew a batch of beer.

    For a typical 20 liter batch of beer this pitch rate could easily be derived to a yeast amount: 18 million cells/ml x 20,000 ml = 360 billion yeast cells.

    Cheers!
     
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  3. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    30 million cells/ml sounds really high.
    Also, I think that 25% yeast solids number sounds pretty low, like a worst case scenario for unrinsed trub. I know Mr. Malty says this number comes from George Fix, but it doesn't seem realistic.

    That aside, 1 Billion x 50% = 500 Million (not 50 million)
     
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  4. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Since you're emphasizing that this is for the case of repitching yeast that was previously used to brew a batch of beer, what would you say is the proper pitch rate in cells/ml/°P for yeast that was not previously used to brew a batch of beer?
     
  5. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    A pitch rate of 30 million cells/ml would ‘correlate’ to a wort gravity of 20 °P (SG = 1.083):

    1.5 million cells/ml/°P x 20 °P = 30 million cells/ml.

    So, if you are brewing a lager like a Doppelbock and repitching yeast that was used to previously brew a beer, then a rate of 30 million cells/ml would be proper.

    Cheers!
     
  6. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Duh. :flushed:
     
  7. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
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    Why would there necessarily be a different rate for "new" yeast? The Mr. Malty page would seem to be talking about there being 4.5 billion cells in 1 ml of yeast solids regardless of new or old, right? From there, isn't it just a matter of determining viability % -- in both "new" and repitched yeast -- to see exactly how many cells are present?
     
    #7 herrburgess, Jul 4, 2015
    Last edited: Jul 4, 2015
  8. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    I tend to agree.
     
  9. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    As Jamil Zainasheff stated: “…the health and vitality of fresh laboratory cultured yeast are superior to yeast harvested from normal fermentation.”

    Cheers!
     
  10. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Sure. But at this point we are talking about the number of total cells, not viability. Isn't a yeast count and viability test required regardless to determine any different pitch rate? Otherwise it's just guess work, correct?
     
    #10 herrburgess, Jul 4, 2015
    Last edited: Jul 4, 2015
  11. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    So what's your recommended pitch rate (cells/ml/°P) for these spectacularly healthy and vital yeast?
     
  12. JackHorzempa

    JackHorzempa Grand Pooh-Bah (3,375) Dec 15, 2005 Pennsylvania
    Society Pooh-Bah

    http://www.mbaa.com/store/Pages/72642.aspx

    I am off to celebrate the 4th of July.

    Happy 4th of July everybody!

    Cheers!
     
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  13. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Happy 4th to you.

    Would you suggest reading this book first, or will a cell count and viability test -- both of which I am planning to do since I will be getting my hands on the necessary equipment -- suffice?
     
  14. Brew_Betty

    Brew_Betty Initiate (0) Jan 5, 2015 Wisconsin

    Can you explain why you feel the need to literally count and quantify the viability of your yeast cells?
     
  15. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Going to be brewing 20 BBL batches (and eventually using the same yeast for 5+ cycles). Want to be on the safe side.... :wink:
     
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  16. TomTown

    TomTown Initiate (0) Feb 7, 2011 Texas

    No offense to you or to any of the users of this site, but if you're planning on opening a brewery and brewing 20bbl batches of anything you probably shouldn't be seeking yeast pitching advice from a site like this. Head on over to probrewer or hit the books for questions like this.

    That said, at the brewery I work for, we generally pitch between 1.8 and 2 million cells per mL/per degree plato to ensure a pretty quick jumpstart on fermentation. Our lagers are generally at terminal gravity in 7-9 days.

    Pitchable slurries that come from yeast labs have crazy high cell densities and are usually pitched at a volume of 1L per bbl yield in the fermentor in lagers under 16 Deg Plato. This link from wyeast does a pretty good job explaining it.

    https://www.wyeastlab.com/com_b_productdetail.cfm?ProductID=14
     
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  17. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Yea, was expecting that response. :wink: Just helping some existing guys out with a recipe with which they are unfamiliar.

    Thanks for the info.
     
    #17 herrburgess, Jul 4, 2015
    Last edited: Jul 4, 2015
  18. TomTown

    TomTown Initiate (0) Feb 7, 2011 Texas

    Sure thing. If you want more info on SOP's/lab practice for counting cells and determining viability, shoot me a message and I'll bore you with as much as you'd like to hear! :slight_smile:

    Unfortunately, I have no way in our current lab setup to determine vitality but if you're brewing on a pretty regular schedule (2-3 day max residency of yeast in a brink) it shouldn't be much of an issue.
     
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  19. herrburgess

    herrburgess Grand Pooh-Bah (3,077) Nov 4, 2009 South Carolina
    Pooh-Bah

    Thanks. Will do that. In the meantime, a question: it looks from the link like the pitchable yeast at Wyeast has 1.2 billion viable cells/ml -- or almost 10x what George Fix (purportedly) says. Is that really the case?
     
    #19 herrburgess, Jul 4, 2015
    Last edited: Jul 4, 2015
  20. TomTown

    TomTown Initiate (0) Feb 7, 2011 Texas

    Yep! These kinds of densities are only really possible when coming directly from a yeast lab though.

    The physical volume of the first generation pitch from the yeast lab will always be less than every subsequent brew because of this.
     
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