Is this starter building worth continuing?

Discussion in 'Homebrewing' started by scurvy311, Jul 20, 2013.

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  1. scurvy311

    scurvy311 Savant (1,135) Dec 3, 2005 Louisiana

    I started with 3 vials of questionable health (hot traveling and best buy date of 8-10-13). I pitched them into 2L of 1.040 on stir plate for 24 hours. I chilled, decanted, then transferred (like I have done 50 times) it to this sanitized glass while making another batch of starter wort. While doing that I see this...(it further settled into 3 distinct layers looking like yeast, dirt, and dirty milk)

    http://s1275.photobucket.com/albums...A1721741_zpsf753ff62.jpg&evt=user_media_share

    I've never seen yeast do this before. Is it worth continuing to build?
     
  2. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Can't say I've seen yeast stratify exactly like that before. What does it smell like?
     
  3. scurvy311

    scurvy311 Savant (1,135) Dec 3, 2005 Louisiana

    It smells normal...bready/yeasty. Should I further decant it? Could it be wort on top, dead cells from vials, then live yeast on the bottom? The middle layer almost looks like suspended trub.
     
  4. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Without seeing the three layers you have now... "yeast, dirt, and dirty milk"... I wonder if the 'yeast' layer (bottom?) is actually dead material, the 'dirt' layer (middle?) is actually healthy yeast that settled, and the 'dirty milk' layer (top?) is spent wort with some yeast still suspended. If so, that's what you'd expect, and I wouldn't worry.

    Edit: meant to ask... does the dirt (middle) layer actually look like dirt, i.e. very dark?
     
  5. Tebuken

    Tebuken Initiate (0) Jun 6, 2009 Argentina

    Let it be for a while to see what happen,wait 3-4 days at 68-72 F, if it doesn´t develop weird stuff then go for it.You should be fine.
     
  6. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Just remembered and found this pic...

    [​IMG]

    That's the best yeast in the bottom layer, so it it can settle that way. Is that what yours looks like now?
     
  7. HerbMeowing

    HerbMeowing Maven (1,295) Nov 10, 2010 Virginia
    Trader

    Nice pix.
    Love homebrew ****.

    I'd add a small volume of sterile water...give it a vigorous swirl to re-suspend everything...chill...and see what settles.
     
  8. HerbMeowing

    HerbMeowing Maven (1,295) Nov 10, 2010 Virginia
    Trader

    Looks like four layers.
    Very bottom one is mostly trub.
     
  9. scurvy311

    scurvy311 Savant (1,135) Dec 3, 2005 Louisiana

    Very close. I'm starting to get the clear wort layer on top like yours. I'll let it settle a little more, decant, then repitch in the starter wort. Thanks for the help, as always.
     
  10. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    In the pic I posted, most of the yeast fell out before most of the trub. It can also happen the other way around.
     
  11. scurvy311

    scurvy311 Savant (1,135) Dec 3, 2005 Louisiana

    So if you had to speculate, based on my pic, which layer would be yeast? Bottom...
     
  12. Tebuken

    Tebuken Initiate (0) Jun 6, 2009 Argentina

  13. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    Are the colors basically the same, but with more defined boundaries than in your pic from early on? If so, I'd say the bottom. The key is where is the creamy white stuff?
     
  14. scurvy311

    scurvy311 Savant (1,135) Dec 3, 2005 Louisiana

    The creamy white stuff is on the bottom. It looks most like what I normally pitch after making a starter.
     
  15. Tebuken

    Tebuken Initiate (0) Jun 6, 2009 Argentina

    Trub is heavier,always.
     
  16. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

  17. Tebuken

    Tebuken Initiate (0) Jun 6, 2009 Argentina

  18. HerbMeowing

    HerbMeowing Maven (1,295) Nov 10, 2010 Virginia
    Trader

    Screwybrewer is the operative word.
     
  19. HerbMeowing

    HerbMeowing Maven (1,295) Nov 10, 2010 Virginia
    Trader

    +1

    Yeast takes time to flocc and drop.
    Trub...not so much.
     
  20. Tebuken

    Tebuken Initiate (0) Jun 6, 2009 Argentina

    Maybe it is not only a matter of weight, trub is not a living thing while yeast it is.
     
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