Some yeast questions

Discussion in 'Homebrewing' started by Zonk, Jul 1, 2016.

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  1. Zonk

    Zonk Initiate (0) Dec 2, 2014 New Jersey

    I recently made a pale ale that I split a gallon out of my 3.5 gallon batch to test ferment with WLP029. Pack was 1 month old and should have had way more yeast than I needed for a gallon, so I boiled and cooled 1.2L of water with 120g of DME cooled and dropped the remaining yeast in there, making a small starter. I did this all late Wednesday.

    I came home Thursday to find vigorous fermentation and high krausen in the 1g jug of pale ale. Erlenmeyer flask with starter didn't seem to have much going on visually (no stir plaate btw, just agitation), but quite a bit of yeast has already settled out. My goal was really just to build up a colony I could use at a later date, so a starter is going to be made from whatever I get from this flask anyway. So my question is, what now? At what point do I crash it, and do I just pour into a sterilized mason jar to store right in the current wort? Secondly, is this now considered 2nd generation even though it hasn't gone through a full fermentation?
     
  2. VikeMan

    VikeMan Grand Pooh-Bah (3,067) Jul 12, 2009 Pennsylvania
    Pooh-Bah

    How long do you plan to keep it? If not for very long (say a few weeks or less), I'd recommend just leaving it in the beer (crashed) until you are ready to decant and start your next starter.

    Why are you concerned about that? Unless you plan to use and re-harvest the yeast through many generations, I wouldn't give it a second thought. I definitely never worry about multi-step starters changing the yeast significantly. Hell, the yeast pack you bought wasn't really a first generation.
     
  3. Zonk

    Zonk Initiate (0) Dec 2, 2014 New Jersey

    I'd like to think I'll use it within a month, but it would be nice to have the option to keep it viable longer, even if it meant I had to fire it up as a starter again. I think 4-8 weeks is probably a reasonable target, but with a 5 and an 8 year old my schedule does spiral out of control on occasion.

    Not really concerned, just like to know how things work. Also I'm just starting out brewing, and at the moment am pretty interested in yeast, and I think I may be trying a bunch of split batches with multiple yeasts. This would mean I'm going to have a bunch of yeasts on hand that I don't necessarily want to be spending $8 per one gallon batch on, so I may be hanging onto some stuff for longer than a few weeks even if it means keeping them viable by firing up new starters. I'll probably never use a strain long enough for it to be an issue unless I find a "house strain," but was just curious if spinning off starter cultures rather than rinsing post fermentation yeast would delay yeast mutation.
     
    #3 Zonk, Jul 1, 2016
    Last edited: Jul 1, 2016
  4. Brewday

    Brewday Zealot (721) Dec 25, 2015 New York

    I would cold crash the flask for two days. Slowly pour the top liquid in one jar and swirl/pour the bottom yeast in another jar. Then fill the yeast jar to the top with the liquid and refrigerate.
     
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